Where can I find Balb/c mouse genome sequence?

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

Look for qualified candidates of Visiting Scholars to Southwest Jiaotong University?

We invite qualified candidates of Visiting Scholars to engage in research collaboration or deliver lectures on their research, with travel expenses covered. The visiting period is flexible,...

20 July 2024 1,165 1 View

How can we effectively assess and monitor the health of the soft and hard tissues around implants so that problems can be detected and treated early?

Although I have extensive experience in dental implants and periodontal treatment, I recognize that we face some deficiencies in my clinic. First, we need to update our equipment, especially in...

09 July 2024 2,004 3 View

What is the association and mechanism between bacterial diversity in the oral microenvironment and clinical manifestations of periodontal disease?

This research question explores the complex relationship between bacterial diversity in the oral microenvironment and the clinical manifestations of periodontal disease. The oral microenvironment...

09 July 2024 6,210 1 View

For 3d transition metal elements, is there a mathematical expression for the energy E in their hard X-ray absorption spectrum?

Only the absorption side is considered, and the fine structure of the extended side is not considered

05 July 2024 2,894 2 View

How to realize charge and discharge of in-situ batteries in PPMS? Is there any expert scholar who can give me some advice?

The difficulty lies in how to miniaturize the battery. The sample holder is only 6.5mm, which means that the width or height of the battery cannot exceed 6.5mm (the height is about 3cm). I hope...

05 July 2024 8,141 0 View

How can technology empower EFL teaching?

Scholars from multiple disciplines stressed the importance of AI Technology in ESL teaching from a theoretical perspective. Are there teaching designs that combine theoretical discussions and...

01 July 2024 999 7 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

How much area/acres of agriculture land can irrigate by 1 cusec of water?

Kindly response

08 August 2024 1,214 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View