Where Can I find an experimental or industrial method for extracting polyphenols by grape pomace?

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Rumen simulation technique (RUSITEC), do you have any contact?

I need the contacts of a company able to furnish or support the set up of a RUSITEC, a continuous cell rumen model. Could do you be able in supporting me? Or, at least, do you have a project of...

25 January 2022 6,115 4 View

In the Thermo Fisher Avantage XPS software, how can we set a specific peak as reference for all other peaks under a narrow scan spectrum?

I have been asked to use the 284.8eV peak in the C1s narrow scan as the reference peak for fitting all other potential peaks for a carbon material. Also, which fitting optimization parameters...

11 January 2022 6,308 5 View

Does anyone know a DNA extraction protocol from starfish?

I tried different protocols but at the beginning of the procedure the tissue (starfish gonads) gelifies and blocks the other extraction phases.

24 May 2021 3,511 1 View

How to calculate response rate if the number of eligible respondents is not available?

Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...

02 February 2021 5,577 3 View

Black leaf-clips (Pocket-PEA, Hansatech) for dark adaptation under high solar radiation underestimate Fv/Fm?

About 10 years ago, I experienced that the black leaf clips used with Pocket-PEA fluorometer (firmly suggested by Hansatech vs. the white version used for Handy-PEA), when used under high...

31 July 2020 5,853 0 View

Can anyone help me with restoring a good DAD baseline?

I have some problem with baseline of my DAD (G1315B Agilent Technologies). I have tried to wash the cell (bypassing column) with isopropanol for a night. The issue seemed to be solved, but during...

10 December 2019 647 0 View

How to report data about studies awaiting classification in a Systematic Review?

Hi All, I am currently undertaking a systematic review. 19 studies were classified as studies awaiting classification. I have contacted the authors. Several authors did not reply and a number...

22 July 2019 4,213 1 View

How should I compare sEMG data across different clinical groups?

Hi All, I am new to the field of sEMG so any guidance or feedback would be greatly appreciated. I am collecting facial EMG data from two groups: healthy vs individuals with neurological impaired...

19 June 2019 6,178 3 View

How should similar studies by the same authors be assessed in a systematic review?

Hi All, I am currently undertaking a systematic review. Two of the studies, completed by nearly the same authors, seem to have used the same set of participants. The differences mainly lies in...

30 May 2019 7,209 8 View

Is there any robust assay to quantify the maturation state of autophagosomes in vertebrates?

I am looking for a way to quantify autophagosome maturation before fusion with lysosomes to see if there is any defect in cargo recognition. I've read about co-localization studies of p62 and LC3...

26 May 2019 2,503 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View