This is type of measurement is not commonly done and I'd be surprised if you find it. However, for MCF-7 here is a place to start(http://pubs.rsc.org/en/Content/ArticleLanding/2014/RA/c3ra46839a#!divAbstract) look in the supple Table 1. Please keep in mind that the sizes are variable and change over passages and in different conditions, so the may not be accurate for your culture.  I would highly recommend you measure your own cells to determine size/volume. Almost all microscope software has measurement capabilities, just make sure you measure at the time of experiment, also trypsinize and measure suspended cells. 

This is type of measurement is not commonly done and I'd be surprised if you find it. However, for MCF-7 here is a place to start(http://pubs.rsc.org/en/Content/ArticleLanding/2014/RA/c3ra46839a#!divAbstract) look in the supple Table 1. Please keep in mind that the sizes are variable and change over passages and in different conditions, so the may not be accurate for your culture.  I would highly recommend you measure your own cells to determine size/volume. Almost all microscope software has measurement capabilities, just make sure you measure at the time of experiment, also trypsinize and measure suspended cells. 

Hi Maria. We have run MCF7 cells on our automated Cell counter (Vi-Cell) in the past. As well as cell number/viability it also gives a read out of average cell size.

From this the volume of the cell can be calculated (as the "size" is the "diameter" of the cell). The cells were Trypsinise so the below value is for the "rounded" cell so volume can be worked out from equation V = 4/3 x pie x radius cubed.

It is worth noting that a cell's size is related to it's health to some degree (I was testing FBS and found that in poorer FBS the cells proliferated less and had a smaller cell size).

Below is readings fro "good" FBS sources.

MCF-7 (in ATCC FBS over 3 passages): 16.4 microns stDev = 1.35 microns

MCF-7 (in Gibco FBS over 3 passages): 16.8 microns stDEv = 1.32 microns.

I hope this helps. Unfortunately I have not grown or analysed HCT116 cells.

Perhaps someone else has ran these cells of a similar cell counter?

Gary

I easily counted my cells using FastRead 102 by BioSigma during seeding of cells.

ARYA SK et al LAB CHIP2012; 12: 2368 gives 18+-2 micrometer diameter.                    It is not specified where from ATCC or else, not specified how many passages, not specified what happens after passage in mice.

MCF-7 surface. I took a note for myself of a number 396-796 micron square section.    No hints about Volume, that for me is THE most impostant.  Perhaps it was from a Hisham Mohammed paper.

MCF7 elastic module.0.3-0,5kPascal double of MCF10A =0.5-1.1kPa. LI QS, 2008.

NOTE breast cancer cell T470 14,8micrometer diam

in vivo Friberg S,  J Surg Oncol 1997; 65: 284 gives 10micrometer the average diam of breast cancer cells. I imagine by optical microscopy: BEWARE diameter observed by MO is quite smaller from that measured by cytofluorimetry.

I need to know diameter and volume of 4T1 triple negative mouse breast cancer cells, GTL16 gastric cancer cell line, _NIH3T3

AND any other cell line

Thank you all. It has been very useful.

Also, searching I have found a paper with measurements of HCT-116 diameter if anyone needs:

M. Tahara et al. / Biochemical and Biophysical Research Communications 434 (2013) 753–759

other measurments! from NCI-60 cell line panel by CELLOMETER, WWW.NEXCELOM.COM          MCF 7 11.95+-3.22 MICRONS it is possible to have

MCF7 clumpy  non clumpy and other versions! So it is mandatory to know where your cells are from. CELL diameter changes over time: at plating they are larger, become smaller (in general) with time.