Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

What is the most accepted probabilistic criterion considering frequency layers (fire) via distribution modeling?

fire index prediction

15 September 2019 3,112 2 View

Could you please suggest some research works about psycology of language?

Dear Sir/Madam, At this moment I'm currently working in my dissertation project. The question is "in which way is the teacher of portuguese as a foreign language conditioned by his culture in...

24 December 2018 5,861 2 View

Minimal number of generators of a k-algebra over commutative rings

08 August 2017 8,849 3 View

MIC concentration and bacterial concentration?

So when using MIC we are basing our numbers established on a bacterial concentration. For instance we say the mic of citric acid for salmonella is 1000ppm. We did the test at a starting bacterial...

09 May 2017 1,870 9 View

The administration of the high fat diet 60% for 10 weeks is efficient in inducing obesity and insulin resistance in C57BL/6J mice?

high-fat 60% for 10 weeks C57BL/6J mice Obesity and insulin resistance

03 May 2017 9,711 5 View

I plaque a 24-well plaq. with C6/36 cells, but after 24h the cells didn't grow up. What can i do to work?

C6/36 cells, Arbovirus. Cell culture. Virology.

23 April 2017 7,869 3 View

Is there a free software available for facial emotion detection confusion?

I am working on my PhD's project. I need to consider emotions of learner, such as confusion and frustration. Is there any free software available for this purpose? I found...

26 March 2017 2,252 3 View

How deep is enough for Ribo-Seq?

I'm working with Ribo-Seq in mouse and I would like to know if someone with experience could tell me how deep in number of reads is enough per sample? Thank's.

28 August 2016 5,898 2 View

Would Capillary sequencing work without previous PCR product amplification?

So my question is base don the following. I have a protein I want from an unsequenced organism. So I went and got the same protein from organisms closelly related and aligned the protein from them...

06 June 2016 9,463 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Is there association of SARS-CoV-2 infection rate with meteorological factors?

There are contradictory research papers about the correlation between meteorological parameters and incidence of COVID-19 in some countries worldwide. Is there correlation between the...

15 February 2021 5,806 2 View