FAME CG analysis (derivation) ?

29 October 2017 9,586 3 View

Does anyone know about the Sevag method for protein removal?

I am extracting Lentinan from Shiitake and the method says that I need to remove protein usin the Sevag method. I have searched and the only thing that I have found is the usage of chloroform and...

03 December 2012 5,187 5 View

How many people/families currently work on informal e-Waste recycling in the world?

A lot of papers and reports suggests that thousands or millions of people or families work as informal "recyclers" in the world, or at least in developing countries. Nevertheless, it is not clear...

01 January 1970 4,906 3 View

*WESTERN BLOT* How do you transfer high and low MW proteins at the same time? I'm trying to get Claudin-5 (~22kDa) and ZO-1 (~220kDa)

I run the electrophoresis in a pre-cast 4-20% gel at 200 V for ~45 min, then i've tried to transfer them to nitrocellulose membrane in the following conditions (always in a cold, wet environment):...

01 January 1970 7,229 6 View

How many people/families currently work on informal e-Waste recycling in the world?

A lot of papers and reports suggests that thousands or millions of people or families work as informal "recyclers" in the world, or at least in developing countries. Nevertheless, it is not clear...

01 January 1970 502 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View