For (non-viral) transfection.
Ok, I found the Addgene and Origene sites.
For example using Ripa buffer? Or will the immunoglobulins fall apart?
10 November 2015 4,253 1 View
I tried staining cytospins of the cell lines for immunoglobulins but frequently there is quite a lot of background. Could I use western blotting?
07 August 2015 2,961 4 View
Farage is described as Epstein-Barr virus-positive, however I cannot detect expression of EBER by in situ hybridization. Can anyone explain this? http://www.atcc.org/products/all/CRL-2630.aspx
10 November 2014 7,436 5 View
I want to induce the lytic cycle of EBV in Akata and Jijoye cell lines using TPA (tetradecanoyl phorbol acetate), sodium butyrate and IgG. I was wondering how long it takes before the induction is...
10 November 2014 6,930 8 View
Detection of EBV-encoded RNA (EBER) via in situ hybridization is the gold standard to determine whether cells contain EBV. I was wondering however whether in EBER-negative cells EBV episomes can...
09 October 2014 9,746 1 View
I do not find any (recent) publications in which such commercially available antibodies are used.
08 September 2014 7,532 4 View
Hi everyone.I've been culturing OCILY3 and OCILY10 cells in RPMI1640 with 20% FBS, 10% FBS or in advance DMEM with 10% FBS (in all cases penicillin/streptomycin and glutamax were added) but in...
06 July 2014 5,404 7 View
Normal B-lymphocytes continuously circulate through the body and although a B-cell lymphoma is not really considered as a solid tumor, it does form a mass. Reading about the epithelial-mesenchymal...
06 July 2014 7,267 8 View
I was wondering if anyone working with lymphoma could tell me which reference/housekeeping genes they include in their PCR experiments?
04 May 2014 452 4 View
Does this methanol concentration kill the EBV particles? Or what would be a better fixative?
07 August 2013 944 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
