Is it possible to extract intact immunoglobulins from fresh frozen immunoglobuline-producing tumor biopsies?

For example using Ripa buffer? Or will the immunoglobulins fall apart?

10 November 2015 4,212 1 View

Can I use western blot to assess immunoglobulin expression in cell lines?

I tried staining cytospins of the cell lines for immunoglobulins but frequently there is quite a lot of background. Could I use western blotting?

07 August 2015 2,901 4 View

I cannot detect Epstein-Barr virus-encoded RNA (EBER) in the Farage cell line?

Farage is described as Epstein-Barr virus-positive, however I cannot detect expression of EBER by in situ hybridization. Can anyone explain this? http://www.atcc.org/products/all/CRL-2630.aspx

10 November 2014 7,374 5 View

How long does the effect of lytic cycle-inducing agents (TPA, sodium butyrate, IgG) last on Epstein-Barr virus (EBV)-producing cell lines?

10 November 2014 6,877 8 View

Can the Epstein-Barr virus (EBV) be episomally present in B-cells without associated expression of viral RNA or protein?

09 October 2014 9,608 1 View

No title

08 September 2014 7,474 4 View

Does anyone have experience with culturing OCILY3 and OCILY10 cells?

Hi everyone.I've been culturing OCILY3 and OCILY10 cells in RPMI1640 with 20% FBS, 10% FBS or in advance DMEM with 10% FBS (in all cases penicillin/streptomycin and glutamax were added) but in...

06 July 2014 5,327 7 View

What is known about metastasis of B-cell lymphoma?

06 July 2014 7,208 8 View

What is the best reference/housekeeping gene for PCR on lymphomas?

I was wondering if anyone working with lymphoma could tell me which reference/housekeeping genes they include in their PCR experiments?

04 May 2014 405 4 View

Is 30-60% methanol (like Thinprep PreservCyt) a safe fixative for EBV-infected cells?

Does this methanol concentration kill the EBV particles? Or what would be a better fixative?

07 August 2013 887 0 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View