How to solve MSTRAT software Running problem?

Hello, we have been trying to run MSTRAT Software for developing a core collection of Chilean Garlic genetic resources, but we keep getting the error: ether couldn't execute...

25 November 2020 9,748 2 View

How to characterize the pore size of membrane filtration with bubble point method?

Hello everyone, I am having troubles with the bubble point measurement to find the pore size of my self-fabricated microfiltration membrane, and I couldn't find a proper answer anywhere else. It...

16 November 2020 7,574 1 View

Is there an estuarine influence in the River Lea (or Lee) in greater London?

I can find very general maps showing the tidal extent in the Thames, but does saline water also travel up its tributaries? Can anyone send me or link me to a map showing the extent of estuarine...

29 October 2020 8,892 1 View

Does any know the half life of Betamethasone in mice?

Hello all, I was wondering if someone could help me. I have read a lot of articles that mention using Betamethasone at clinically relevant levels in mice yet no one has mentioned the half-life. I...

08 September 2020 2,255 2 View

Can I use ANOVA with percentage data?

I am looking at the association of two molecules within a cell (expressed as a %association of one with the other). I have looked at this association at three different time points (i.e 6h, 12h,...

01 April 2020 510 4 View

Does any one know any auditory and optic Tests for mice?

Hello there, Before I begin my experiments, I would like to make sure all my mice in my experiment have proper vision and hearing. However the only test I know that would work is administering a...

19 February 2020 5,766 2 View

I'm having problems with Superoxid Dismutase assay. Is there anything I can change in the metodology?

I've been studying plant metabolism and in my lab we ar used to measure superoxid dismutase activity using the methodology of Giannopolitis e Ries, (1977), using the nitrotetrazolium blue...

17 December 2019 7,272 1 View

Does anyone have any insight into researching intermittent fasting and calorie restriction in relation to oxidative stress in the nervous system?

I am interested in researching intermittent fasting (IF) and caloric restriction (CR) in relation to hypothesized positive effects on oxidative stress in the nervous system, specifically in the...

09 September 2019 7,012 3 View

No title

I am trying to create a FIS fuzzy logic file on Matlab. I am referring myself to a pdf which has the rules and triangle form of the membership. The display range of the function is from - 0.2 to...

09 August 2019 8,016 6 View

Precipitated/concentrated protein not dissolving in RIPA Buffer, what can I do next?

Hi everyone. I tried concentrating and precipitating my protein using ProteoExtract Protein Precipitation kit. I did not have any of the buffers suggested to dissolve so I used RIPA buffer....

26 February 2019 3,141 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View