Could anyone suggest me why I am not getting PCR bands for UTR region?

I am trying to amplify UTR region of a gene by desiging primers against genomic sequence. Melting temperature for all primer sets is 59. I tried with different master-mixes (Robust, emerald, Fast,...

26 July 2016 7,169 3 View

Any advice on the problem in Capacitance calculation from Cyclic Voltammetry (CV) data ?

I want to calculate specific capacitance. So first i have to find area under curve. I have used trapezium rule for that as u can see in attachment. i have subtracted area under curve from point...

24 August 2015 4,761 13 View

How do I use lectins on tissues?

Ulex europeus agglutinin - 1 use in tissue histochemistry?

01 February 2015 9,903 5 View

Why is my DNA degrading after storage?

I am getting good bands after DNA isolation but not getting PCR results. Also, my DNA degrades after storage. What could be the problem?

26 March 2014 3,127 10 View

How can bioactive form in bioactive borate glasses in the SBF solution ?

I'm working on bioactive glasses(Borate glass). There was not HAp layer formation deducted in XRD after immersion in SBF(simulated body fluid) solution. I immersed powdered sample for 12...

01 January 1970 4,608 4 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View