The identification of a protein differed based on RAST and protein BLAST results. Is RAST reliable? Or does this mean that both are correct and the protein has both identities? Eg it is a subunit in two different but related enzyme complexes?
Hello. I can´'t find a full text article that i need for my research. Can you help me? The reference of the article is: Ref ID: 3441009. Article: Adolescent Health: Communication With Adolescent...
16 May 2024 3,293 3 View
Hi everyone! As I previously shared with you, I've started to work with Caco-2 cells and even asked for your help. I recently followed a protocol to extract proteins, but when I collected the...
12 May 2024 6,819 2 View
Hello! I am currently running a western blot using human AD tissue samples. I prepared these samples over a year and a half ago. I am having trouble standardizing these proteins with Beta Actin. I...
02 May 2024 9,588 3 View
I am currently running a plasmid isolation. I am using a bacterial stab to grow the plasmids. We have used this stab a few times now. Unfortunately, we have not had the chance to make glycerol...
08 April 2024 2,035 4 View
Hello! I have carried out my undergraduate research project using two pre-validated, Likert scale self report questionnaires looking at emotional intelligence and resilience. (SSEIT and...
16 March 2024 7,642 3 View
Hi, I am carrying out a very basic undergraduate research study with no stats teaching on the course (medical degree) as a result I am floundering and wonder if anyone could help. I am using two...
01 March 2024 6,741 9 View
I am currently working with HT-22 cells by transfecting them with bacterial plasmids to perform IHC. However, we have not had much success with reaching a 50% transfection rate. I usually do about...
17 January 2024 7,802 6 View
hi again! I had never worked with Caco-2 cells before and just recently I thaw some. When I look at them I see something that I really don't know what it is. I know these cells secrete a lot of...
14 January 2024 9,068 4 View
Hello. I plan to do a simple immunofluorescence procedure with HT-22 cells. I plan to transfect the cells and fix them. I do not believe I will have time to come in on the weekend. Is it possible...
11 December 2023 6,640 2 View
Hello! Does anyone have experience in solving the issue of leaking due to the film not fully adhering, after following all the instructions from...
19 October 2023 618 0 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View