The identification of a protein differed based on RAST and protein BLAST results. Is RAST reliable? Or does this mean that both are correct and the protein has both identities? Eg it is a subunit in two different but related enzyme complexes?
As you all know, this pandemic has taken a major toll on the entire global community. In some societies, people do abide by COVID protective measures. Yet, in other societies people are resistant...
30 September 2020 546 13 View
I am trying to clone using the pcDNA3.1 V5-His-TOPO expression kit. There is no problem transferring it to the cell, but I can only transfer the vector. I did not succeed in vector-insert...
06 September 2020 3,483 0 View
I have included pictures to illustrate my point. In the gel image that has only 4 lanes: Lane 1 is a 1 kb molecular weight ladder. Lane 3 is my wild type DNA (in a pGEX plasmid) and Lane 4 is the...
28 August 2020 4,146 4 View
I analysed SARS-CoV-2 Spike Protein delta Furin mutant in my bachelors thesis and need evidence (paper, research or something like that) to proof my suggestion. I think the double band at around...
26 August 2020 1,058 4 View
I apologize in advance for my rookie question. I have been looking in the internet how to get SD from CI values, and some sources tell me to apply the formula =SQRT(N)*(CI_upper.limit -...
19 August 2020 8,946 2 View
The BREQ-2 tool is commonly used in physical activity research to measure motivation. The tool was developed by David A. Markland. I have not been able to find how to ask consent for its use in...
15 April 2020 5,428 3 View
So for example, we have the following polls: First poll: People who said they like orange juice for breakfast are as follows: Texas (50%), NY (25%), North Carolina (20%), and Florida (5%) Second...
01 March 2020 2,916 3 View
I have been recently studying proprietary voter files and data. While I know that voter files are for the most part public (paid or free), I am confused as to how companies match this data to...
30 January 2020 7,520 1 View
Hello, Here is what I got with Bowtie2 while changing .sam file to .bam file: "samtools view -bS egpart1.sam > egpart1.bam [E::bgzf_flush] File write failed (wrong size) samtools view: writing to...
28 December 2019 3,244 2 View
I am asking for a student I'm mentoring. It's an action research project with a small sample of maybe 8-10 participants. If the student includes a likert attitude scale but is mostly focusing on...
08 June 2019 9,553 4 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...
02 March 2021 752 2 View