Melissa Yeow

5 Questions 4 Answers 0 Followers

Questions related from Melissa Yeow

Is there an efficient way to search for particular enzymes in whole genome sequences?

I have whole genome data in fasta format, and the gene/protein sequences of the target enzymes. I've tried BLAST for 2 sequences, but the results are strange, possibly because the whole genome...

19 December 2018 1,368 2 View

Has anyone tried the EchoCLEAN TM CleanUp Kits from BioEcho Life Sciences for DNA cleanup? How is the efficiency like?

I've been having trouble with purifying my DNA post-PCR using gel extraction techniques as I lose all my DNA and have a 230nm peak which may be contamination by ethanol of guanidinium salts. Any...

04 December 2018 8,566 0 View

Trouble purifying PCR product using Qiagen gel extraction kit and Monarch gel extraction kit, 230nm peak?

I start with impure PCR products of >500ng/ul, 260/280=1, but peaks present at 260, and end up with peaks near 230, about 10ng/ul concentrations of 260/280=1.5 or so, which I assume is not DNA...

30 November 2018 6,899 8 View

What is the best way to purify DNA during SOE PCR?

According to protocols I found from Nature (Heckman et al 2007) and Cold Springs Harbour (Forloni et al 2018), the PCR products must be purified using gel electrophoresis and then a QIAquick gel...

06 November 2018 4,789 9 View

When the RAST labelling of your protein is different from the results of your BLAST search, how do you resolve this conflict?

The identification of a protein differed based on RAST and protein BLAST results. Is RAST reliable? Or does this mean that both are correct and the protein has both identities? Eg it is a subunit...

17 September 2018 4,467 0 View