When precipitating phage and taking image phage tails are getting broken, how can we get a complete phage image with its tail?

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Michael J. Benedik

For many phages you can use PEG precipitation and fairly gentle centrifugation. I think high speed centrifugation may be what inactivates the tail.

However the best method would be equilibrium gradient ultracentrifugation so that you never pellet the phage.

Achyuta Nanda Sahu

Thanks will see.