When making buffers, it seemed that the buffer solution's PH bounce a little bit when put overnight even the reading was stabilized from PH meter the previous day.
The pH of bicarbonate solutions tends to increase over time, presumably due to evaporation of CO2, so this solution should be made fresh each day. Buffer pH is also temperature-dependent.
1- Buffers are solutions which can resist changes in pH when acid or alkali is added.
2- H2CO3 / NaHCO3 (Bicarbonate buffer) (carbonic acid and sodium bicarbonate).
3- The pH of bicarbonate solutions tends to increase because the evaporation of CO2.
4- Bicarbonate buffer should be made fresh each day. I am agree with Adam s B Shapiro answer ..
5- Buffering capacity its depend on the concentration of the buffer and the relationships between the pKa of the buffer desired pH.
6- The buffering capacity is determined by the actual concentrations of salt and acid present, as well as by their ratio.
7- Bicarbonate buffer in the body (pKa = 6.33, concentration = 25).
Thank you Dr. Moaed E. Algazallyand and Dr. Adam B Shapiro, your answers greatly solved my confusion.
In case you are concerned with the carbonic acid (H2CO3) / sodium bicarbonate (NaHCO3) buffer, you may possibly want to check the following discussion: https://www.researchgate.net/post/How_can_stabilise_the_pH_of_01M_sodium_bicarbonate_solution_made_in_the_lab_to_80
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