It can be found in most literatures and reviews that METTL3 exerts its methylase activity in the nucleus, interacting with adaptors such as METTL14 and WTAP, but is it possible for METTL3 to play the role in the cytoplasm?
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
Hi all, I am trying to use mixed effect model to analyze my data, which including a baseline measurement for my exposure (A), and repeated measurements for the outcome (B). I do have some...
17 July 2024 8,682 3 View
Dear colleagues, As well known, we can deliver messages in different languages and characters, including Chinese, English, Latin, binary and decimal codes (if with a converter) and many other...
08 July 2024 5,422 13 View
Hello, I am experimenting to grow cells onto permeable membrane support (inserts) in a 12-well plate. The cells are stained with DAPI. Just wondering how to image the cells attached to the inserts...
20 May 2024 1,061 0 View
Use of thioglycolic acid as a stabiliser during the synthesis of nanoparticles
05 May 2024 9,109 1 View
Now I need to carry out the task of frequency calculation on a large number of models, because only one CPU per job for frequency calculation, so in order to improve efficiency I have to submit...
09 April 2024 652 2 View
This is the first time I did an H&E staining, and I found that there are some white (blank) spaces between the cells. I do not think this is the normal morphology of this tissue. Here are...
08 April 2024 8,491 2 View
I am an international and a first-year undergraduate Medical Science student at UNSW. How can I get involved in research as soon as possible, and if I can't, what should I do to ensure that I am...
29 March 2024 4,934 1 View
Hi all, I am new to the computational suite"MolPro" and I am trying to do the Electronic energy calculation for a small system. Following is my Input...
29 March 2024 1,178 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View