It can be found in most literatures and reviews that METTL3 exerts its methylase activity in the nucleus, interacting with adaptors such as METTL14 and WTAP, but is it possible for METTL3 to play the role in the cytoplasm?
In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...
21 February 2021 1,864 2 View
I would like to know in the oil extraction, which is more efficient: gas flooding or water flooding? Will water flooding be more efficient in hydrophilic reservoirs with capillary...
29 December 2020 3,471 3 View
I measured different kinds of catalysts which were dispersed in DI water.I firstly added certain amount of HCl and then used NaOH to titrate and measure the pH value. What does this result mean?
27 December 2020 5,728 4 View
Dear scientists, I have a question to ask you, it is known that Mn4+ is beneficial to the soil, but part of Mn4+ in the soil is easy to be converted to MN2 +, is there any way to inhibit its ion...
21 November 2020 1,200 5 View
02 October 2020 8,642 1 View
We obtained the global results using remote sensing reanalysis dataset. But the snow-coverd areas should be removed (like the blank area in the picture below, sources: Purdy et al., 2016). Does...
20 September 2020 3,463 5 View
I use fluent to simulate species transport. The medium is sulfur hexafluoride and air. There is a density item under the mixture, and there is also under the fluid. Is there any difference between...
29 July 2020 7,414 4 View
During aging or storage, the concentration of monomeric anthocyanins decreases rapidly, while low level of polymeric anthocyanins generates, and the color of wine turns brick-red gradually. In...
17 July 2020 6,032 4 View
As is shown in attachment, there is hybrid plasmonic waveguide (HPW) and dielectric waveguide (DW), I want know how to calculate mode overlap ratio to evaluated the mode distribution similarity...
14 July 2020 2,275 3 View
Since that my experiment could not be proceeded in aqueous. Anyone who know is there any organic solvent for MnCO3, the concentration could be as low as several mg/mL. Thanks
02 July 2020 9,654 19 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
02 March 2021 3,060 3 View
02 March 2021 7,082 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View