I am working on flow cytometry of intracellularlly stained samples gating for single and viable cells. Does time gating help improve selecting population of interest? I would appreciate if someone explain me how to do time gating.
Time parameter helps you to observe that your cells are consistently flowing, as an internal control. It can also help you to see kinetic changes in your sample. Using time parameter depends on what you would like to see, you may apply Viability/TIME to observe if your cells are viable or you may decide to see how your specific set of cells, such as CD4 T cells, are flowing/changing in TIME.
Again gating also depends on what you would like to see, if you set your gates for certain time windows, you will explore the changes.
Time parameter helps you to observe that your cells are consistently flowing, as an internal control. It can also help you to see kinetic changes in your sample. Using time parameter depends on what you would like to see, you may apply Viability/TIME to observe if your cells are viable or you may decide to see how your specific set of cells, such as CD4 T cells, are flowing/changing in TIME.
Again gating also depends on what you would like to see, if you set your gates for certain time windows, you will explore the changes.
This article might be of interest to you, item 2 on the list is about the time gate
https://expertcytometry.com/3-flow-cytometry-gates-that-will-improve-the-accuracy-of-your-facs-data-analysis/
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