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Questions related from Stephan Spangenberg
I have total RNA extracted from samples which are rather precious. I am trying to perform cDNA synthesis then qPCR. The RNA appears to be intact and reasonably pure, but I only have between 20 and...
03 March 2019 7,615 4 View
Hello Research Gate, I am planning on using a quite specific cell line which has been immortalized with the temperature sensitive SV40 T antigen. Cells immortalized this way are widely reported to...
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I have a CRISPR/Cas9 Wild Type (pSpCas9(BB)-2A-GFP (PX458)) plasmid that I have prepared, targeted for a nuclear protein's gene. I have already confirmed its sequence. I would like to test if it...
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I am attempting to produce lentivirus vectors using the pLKO.1 system according to the following protocol: http://www.addgene.org/tools/protocols/plko/ In this protocol, HEK 293T cells are...
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I cut a MSCV vector with NotI and SalI. The vector is 6kb long and the restriction sites are about 100 base pairs apart. Upon performing gel electrophoresis, the cut vector produced a clear,...
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