When experimenting using flow cytometry and caspase 9, how are the compensation, positivity thresholds, and strategy determined?

4 weeks ago 1 6K Report

Jahaziel Gasperin Bulbarela

Using compensation beads or antibodies to other positive markers in the cell with same fluorochrome that your caspases antobodiese. You need an induced-apoptosis control like Camptothecin. There are kits to detect caspases where you can find relevant information.

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