I am trying to isolate total RNA from yeast lysate. The lysis is done using glass-bead beating. The lysate is collected and precipitated overnight with sodium acetate and ethanol. The precipitant is resuspended in trizol and chloroform, spun, and supernatant (aq phase) is collected and precipitated using isopropanol. But for random samples and isolations, I am getting thick 5.8/5s and faint higher bands. I does not look like a typical degraded RNA which usually appears like a smear? Does anyone has any explanation as to what might be going wrong? I am confident about the reagents used and other glass wares/plastic wares used during isolation that they are RNAse free (I have controls for those). I have attached two images for reference. What might be happening?