I am using iPSC-derived macrophages and am looking for LC3 levels as an indication of autophagy. I'm using rapamycin as an inducer of autophagy and was wondering when (or if?) I need to add bafilomycin? It seems like published literature either adds bafilomycin 1 hour before harvesting at 100nM (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614970/) or 2hour following treatment at the start at 400nM(https://www.mdpi.com/2073-4409/10/1/95/htm). Additionally, how long should I induce with rapamycin?