Priyadarshini Chatterjee

7 Questions 3 Answers 0 Followers

Questions related from Priyadarshini Chatterjee

Can you store a cytokine at a lower concentration than manufacturer's instructions?

We have ordered mouse IL1beta from Thermo for a T cell differentiation assay and the recommended storage concentration is 0.1-1mg/mL. Would it be okay to store it at a lower concentration of...

27 June 2025 7,937 1 View

Fisher's LSD vs Tukey's HSD test?

I am looking at the effect of stress on a number of stress genes via a qRT PCR. I have put them into graphpad where my groups are +/- the chemical inducing stress and each row is one gene - all...

31 December 2023 229 0 View

2-way ANOVA using an interaction term versus not: getting different p value significance?

Using GraphPad Prism, when I carry out a 2-way ANOVA with an interaction term, data between two groups is not significant. The post-hoc tests shows non significant interaction between my column...

25 December 2023 7,203 3 View

Methods to find RNA binding proteins?

Hi, What are some easy methods to find RNA binding proteins? Best, Priyadarshini

30 June 2023 1,916 2 View

How important is it to add BafA while studying autophagy?

I am working with iPSC-derived macrophages and looking at autophagy in these cells. Rapamycin induces expression of LC3II while bafilomycin prevents its turn over. From my data (and previous data...

11 January 2023 2,361 1 View

When do I add bafilomycin A1 following rapamycin treatment to my cells for a LC3 read out assay?

I am using iPSC-derived macrophages and am looking for LC3 levels as an indication of autophagy. I'm using rapamycin as an inducer of autophagy and was wondering when (or if?) I need to add...

04 August 2022 2,675 0 View

How do I show that different cell line growth is statistically significant?

I'm comparing the growth of two different cell lines across a fixed time period - what sort of test do I do to show that they are statistically significant (or not)? Would I do a 2 way ANOVA as...

27 March 2021 1,770 3 View