Hi,
I would like to treat differentiated C2C12 myotubes with a toxin during 3 days. For differentiation I only renew the medium every 2 days (or 3 days on weekends) After differentiation should I renew the medium every day ?
Thanks for your help
Paul Hafen
After differentiation we continue to change the media every other day (48 hrs)... also, we never go 3 days between changes... if the 48-hr media change falls on a weekend, then we come in for a quick media change on the weekend...
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