When culturing human colon organoids, is it better to use B27 with or without retinoic acid?

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Issues with Permeabilization during Flow Cytometry?

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Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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Juan Pablo Peña Ruiz Esparza

Definitely you need the B27 plus retinoic acid because this and others short chain fatty acids is the more natural way to feed the colonocyte to behave naturally in lab.

Jana Jezkova

I've now received a response from R & D systems' technical service:

"I believe that working in Vitamin A Free conditions is necessary for neural stem cell expansion as limited and controlled retinoic acid production reduce unwanted NSC differentiation. I do not think it is necessary to use Vitamin A Free Media Supplement when culturing human colon organoids. I believe that in the case of those few protocols were vitamin A free material was used with colon organoids it may have been that it was available and therefore used but not strictly necessary. To be sure I am giving the correct answer I am checking with the people that develop the culture media and supplement and I will let you know more soon as possible also about the N2."