I am interested in the dynamics of the cellular immune response during Leishmania infection, particularly with regards to immune cell recruitment to the site of inoculation. What is the earliest time-point at which antigen-specific CD4+ T cells are present at the site of inoculation? Could you recommend any papers which have used flow cytometry or imaging methods to investigate this in mouse models?
Yasmine Belkaid has published multiple papers as first and senior author on the nature of the T cell response during L. major infection.
Figure 5 in this paper shows ag-dependent production of IFNg and IL-10 across a time course:
Published November 19, 2001 // JEM vol. 194 no. 10 1497-1506
The Rockefeller University Press, doi: 10.1084/jem.194.10.1497
© 2001 Rockefeller University Press
Original Article
The Role of Interleukin (IL)-10 in the Persistence of Leishmania major in the Skin after Healing and the Therapeutic Potential of Anti–IL-10 Receptor Antibody for Sterile Cure
Yasmine Belkaid 1 , Karl F. Hoffmann 1 , Susana Mendez 1 , Shaden Kamhawi 1 , Mark C. Udey 2 , Tom A. Wynn 1 , and David L. Sacks 1
Trends Microbiol. 2003 May;11(5):210-4.
Neutrophil granulocytes--Trojan horses for Leishmania major and other intracellular microbes?
Laskay T1, van Zandbergen G, Solbach W.
PMID: 12781523 [PubMed - indexed for MEDLINE]
Around 2 weeks after infection.
IL-4 Attenuates Th1-Associated Chemokine Expression and Th1 Trafficking to Inflamed Tissues and Limits Pathogen Clearance
Christopher A. Lazarski,1 Jill Ford,1 Shoshana D. Katzman,1 Alexander F. Rosenberg,2 and Deborah J. Fowell1,*
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