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I proliferate Clostridium butyricum, pasteuricum, and tyrobutyricum in RCM at 30 degrees Celcius for 3 days. They all look very turbid. Then I dilute them in 1X PBS and plate them onto RCA. The...
08 November 2020 6,919 4 View
My Clostridium tyrobutyricum seems not producing spores. I incubated the Clostridium tyrobutyricum in 10 ml of RCM at 28 degrees Celcius for 5 days anaerobically, then heat the whole tube in 75...
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I spiked in 4-8 log CFU/ml of Lactococcus lactis into milk, and use DNeasy Powered Food Kit to extract DNA from it. Nanodrop 2000 was used to determine the values. The yield and quality are weird:...
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