Lim Wei Gene

6 Questions 19 Answers 0 Followers

Questions related from Lim Wei Gene

Oxacillin binds to which peptidoglycan synthesis protein?

Hi all. As we know Oxacillin antibiotic drug inhibits peptidoglycan synthesis in both gram-positive and gram-negative bacteria. Does anyone know which particular protein does Oxacillin binds to in...

02 December 2021 8,645 4 View

Which peptidoglycan protein does oxacillin binds to MRSA?

Hi all. When I did molecular docking on oxacillin antibiotic against PBP-2A (1VQQ), the oxacillin does binds to the PBP-2A protein, and I selected the lowest free energy bind for further analysis...

29 November 2021 1,422 0 View

What protein in Aeromonas hydrophila is targeted by Ceftazidime antibiotic?

From my understanding, Ceftazidime antibiotic inhibits the peptidoglycan synthesis process by binding to penicillin-binding protein. But in Aeromonas hydrophila I could not find any...

19 November 2021 6,336 4 View

Is there another way to determine the anti-quorum sensing effect via quantification of violacein production?

If the compound shows growth inhibition of C. violaceum, can I still proceed to determine the anti-quorum sensing effects? How can I quantify the production of violacein production?

09 September 2021 4,880 2 View

How can I permeabilized gram-negative outer membrane in order to stain the peptidoglycan layer using WGA stain?

I would like to determine if the novel drugs target peptidoglycan synthesis on gram-negative bacteria cells by staining with WGA stain, which stains the peptidoglycan layer. But the main problem...

25 May 2021 1,894 1 View

Concentration of lysozyme and H2O2 in phagolysosome of THP-1 macrophage cells?

I would like to know whats the concentration of lysozyme and H2O2 in the phagolysosome of THP-1 macrophage cells or in general human macrophage cells. Any idea?

01 January 1970 7,631 4 View