What's the relationship between PH and medim dregs?

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Hayat Essalmani

pH could solubilise or insolubilise the molecule. On your case you have precipitation in your medium after ajustement of the pH

Shiva Mojarrad

Dear Hayat Essalmani

thanks for the answer.yes there is precipitation after pH adjusting, but I'd like to know the reason and solve it.

use a buffer and a pH value that does not precipitate your medium

dear Hayat

thank you. which kind of buffer do you recommend?

Ricardo Julian Licea-Moreno

Have you checked the variations or established the dinamic of variations of pH in culture medium?

Dear Ricardo Julian Licea-Moreno

Thank you very much for the answer

I have adjusted pH on 6.7 before and had the same problem. Should I try more pH variations?

My question is in other way. Since there's not dregs without adjusting pH, probably the pH that you're using for your cultures is not "suitable" for formulation. For the preparation of culture medium, are you using commercial dry power or stocks solutions?

I'm sorry for misunderstanding

yes we usually use dry powders of SIGMA company

I will test the medium with different pHs and check the results.

thank you very much for the time and answer

It's interesting that after adjusting the pH, appear dregs. In some cases I have observed dregs after autoclaving, drastic changes in pH and/or after several days of culture. By now, I only can recommend to check the caducity of dry powder and/or water quality.