I am working on cryopreservation of 'Echinacea purpurea' hairy roots , but my hairy roots all die. the medium of hairy roots (liquid WPM) has dregs and the color of the medium is conescent. Recently I have noticed that after I adjust PH to 5.8 (using KOH or NAOH and sometimes HCL) these dregs appear, but without adjusting PH there is no dregs and the medium is clear like water.
Can anyone let me know the scientific reason and advice for solving this problem, please?
Many thanks
pH could solubilise or insolubilise the molecule. On your case you have precipitation in your medium after ajustement of the pH
Dear Hayat Essalmani
thanks for the answer.yes there is precipitation after pH adjusting, but I'd like to know the reason and solve it.
Have you checked the variations or established the dinamic of variations of pH in culture medium?
Dear Ricardo Julian Licea-Moreno
Thank you very much for the answer
I have adjusted pH on 6.7 before and had the same problem. Should I try more pH variations?
My question is in other way. Since there's not dregs without adjusting pH, probably the pH that you're using for your cultures is not "suitable" for formulation. For the preparation of culture medium, are you using commercial dry power or stocks solutions?
Dear Ricardo Julian Licea-Moreno
I'm sorry for misunderstanding
yes we usually use dry powders of SIGMA company
I will test the medium with different pHs and check the results.
thank you very much for the time and answer
It's interesting that after adjusting the pH, appear dregs. In some cases I have observed dregs after autoclaving, drastic changes in pH and/or after several days of culture. By now, I only can recommend to check the caducity of dry powder and/or water quality.
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