What's the max. passage on HSKM cells / can they be grown/split/differentiated similarly to C2C12 cells?

05 May 2016 1 822 Report

We are considering a move from C2C12 cells to HSKM cells since the former is not applicable for GLUT4 studies, however we don't have any experience with human cell lines. My questions are:

1. What is the max. passage possible on HSKM cells?

2. Can they be grown / split / differentiated similarly to C2C12?

3. Are there any issues regarding their expression of GLUT4 or Insulin Receptor?

Thank you in advance,

Stefan

Wai Chin Chong

max 20 passages. After that, the cell tends to have altered morphology

Badges
Science method

More Stefan Kühn's questions See All

How can I best determine extracellular (media) glucose concentration of differentiated C2C12 cells (non-radiometric)?

I'm trying to measure the extracellular glucose of my cells to get a feel for their rate of glucose uptake (from a fixed concentration of glucose), however I'm not really an experimentalist and am...

31 December 2015 8,195 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View