There is not a single marker for NKT cells.

A combination of CD3 and CD56 is most used but not 100% proof.

T= CD3+CD56-

NK= CD3-CD56+

NKT = CD3+CD56+

Thanks Ronald

What I know that, also some NK cells can be CD3CD56 douple positive.

How can we differentiat this group of cells from NKT group which is also CD3CD56 douple positive.

Also today I have read that there is TCR V24 alpha 18 beta on NKT cells, can I use it also as a specific marker for NKT.

Hi, here you'll find a full gating strategy guide for NK(T) cells: http://cytobase.montp.inserm.fr/ftp/Phenotyping%20NK%20cells%20and%20NKT%20cells.pdf

Thank you Dominik

Do I need also to look for TCR V24 alpha 18 beta to confirm that it's NKT cells.

Hi, they are CD161 , CD56. CD3, CD1a, CD16

Thanks Emmanuel

For the aGalCer/CD1d tetramers in detection of NKT cells, how is the gating strategy is going to be?

Check this link may be helpful

https://www.biolegend.com/natural_killer

Regards

Thank you so much

CD3 , TCR(V-alpha24 or V-alpha24-j-alpha18) with V-betta11

V alpha 24 can only be used for isolation and detection of invariant NKT, which is type I NKT cells, not the whole population of NKT cells