Afshin Derakhshani

5 Questions 9 Answers 0 Followers

Questions related from Afshin Derakhshani

Why are there multiple overexpressed protein bands in my SDS PAGE?

I have cloned a gene in Suffle T7 express strain using pET28a vector for expression and in vitro studies. After Ni-NTA column purification of this His Tagged protein, I am getting multiple bands...

02 February 2019 8,641 6 View

what is the best method for protein extraction from tissue?

I wanna detection of IFN-gamma and IL4 from human tonsil what is the best method for protein detection ?

07 July 2018 2,494 3 View

Can we use mixture of oligo dt and random hexamer for synthesis cDNA?

Is a random primer or oligo(dT) better for RT-PCR reaction? and Can we use them together

06 June 2018 7,531 3 View

How can I measure a gene with a very low expression by qRT PCR?

I wanna detection genes related to NKT cells (receptors), because of these cells are so low in our blood .detection of these genes is so hard and when I try by SYBR® Green Real-Time PCR, My...

06 June 2018 9,695 11 View

What is the reason for lines in separating gel?

I have a big problem with my SDS gel, When I run my proteins ,I see a line during running and after Staining and ,Destaining I have a line whole of my gel . I attach some photos of my gels.

06 June 2018 1,809 5 View