Recently check the cell lines, 60% of the cell lines are infected with Mycoplasma. Some common antibiotics for treating Mycoplasma were used to treat cells for 3 weeks, but mycoplasma is still there. We also bought some kit, the Mynox Mycoplasma Elimination Kit from Sigma. However, one kit is only for one cell line, but we have so many cell lines and that would cost thousands.
Is there any good way to eliminate Mycoplasma and be cheap?
I checked some documents showing surfactin is good. Anyone know this reagent?
For resistant mycoplasmas, you can try Plasmocure from Invivogen:
http://www.invivogen.com/plasmocure
Not so expensive and very efficient.
Hi,
It maybe due to incubator contamination, so I suggest that removing all plates in the incubator and washing its using Sodium hypochlorite and try again cell culture .
Surfactin is a strong antibiotic agent, already used in vaccines, and shows antimycoplasma properties. Besides, it is a natural lipopeptide with low toxicity in in vivo models.
Check Vollenbroich et al.
"Antimycoplasma properties and application in cell culture of surfactin, a lipopeptide antibiotic from Bacillus subtilis"
http://aem.asm.org/content/63/1/44.full.pdf
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