I'm looking for the best performance to extract RNA from some tissues which are stored for more than 1 year or even a couple of years in - 80 degrees of Celsius and we are not sure about its safety in storage.
Given the time of storage, you might expect to have some loses, so provided you have at least 50mg of fresh tissue I would suggest a quick and easy Trizol extraction kit protocol.
They are easy, fast and efficient with the ability to extract even small RNAs. Make sure you aliquot your samples and use liquid nitrogen to keep them cool at least until the Trizol step. Here's a "sample protocol". Remember each company has minor differences on their protocols, so make sure you follow directions exactly.
http://tools.lifetechnologies.com/content/sfs/manuals/trizol_reagent.pdf
Best of luck!
thank you all ...
but for some reasons I prefer to use RNA x plus to extract...
Hi Aref
I extracted RNA from plant tissues which are stored for more than 7 months in - 80 degrees by using RNX-plus and I got good results. CinnaGen company protocol was used.
hi mrs.matlobi
thank you for your reply ... I'm using old tissues which are stored for more than 2 years in -80 degrees of Celsius and every time I had tried to extract the RNA I had a problem ... and even some times I lost my RNAs totally ...
Isopropanol must be cold. was it in -20 degree for 12 hours at least?
It is very important!
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