I need a positive control to compare it to mesenchymal stem cell TLR3 and TLR4 expression.
You can try:
THP-1
U937
They are only monocytic, not exactly macrophages in their undffierentiated state. But their differentiation has been extensively documented in the literature.
http://www.nature.com/nature/journal/v306/n5943/abs/306597a0.html
http://www.nature.com/nature/journal/v512/n7515/fig_tab/nature13684_SF4.html
http://www.atcc.org/products/all/TIB-202.aspx
Both are suspension cells.
Thank you very much for your answer.
Best regards
If you want macrophages, treating THP-1 with PMA 48H prior to the measurement is also good prior to the analysis. Good luck!
I agree. Optimized THP-1 differentiation can be achieved with very low dose PMA.
Please see this methods paper.
http://www.ncbi.nlm.nih.gov/pubmed/17334670
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