Does anyone know what's the Lin(–), c-Kit(–), Sca-1(+) population of murine hematopoietic progenitors?
I use murine HSCs and sort them by FACS, and of course, my major interest is Lin(–), c-Kit(+), Sca-1(+) population. I tried to understand what's in other 3 populations gated by c-Kit and Sca-1 positivity. I could find the information about 2 of 3 population, but c-Kit(–), Sca-1(+) population wasn't on the list of progenitors.
Does that mean nobody knows what those cells are??
Please e-mail your question to my earlier colleague [email protected]
Dr. Zhang has done lot of work in this area in mouse, he will answer your question
Subhash C. Juneja
Thank you so much. As you recommended, I'll write him an e-mail.
Best regards,
Daisuke
Hi,
Until then, here is some information of relevance:
https://www.stemcell.com/flow-cytometry-methods-for-identifying-mouse-hematopoietic-stem-and-progenitor-cells.html
http://www.bloodjournal.org/content/bloodjournal/80/12/3044.full.pdf?sso-checked=true
https://www.jimmunol.org/content/181/11/7507
Article The EndoglinPositive Sca-1Positive RhodamineLow Phenotype De...
Article Cardiac Sca-1+ Cells Are Not Intrinsic Stem Cells for Myocar...
https://www.biorxiv.org/content/10.1101/590679v1.full
Article CD201 and CD27 identify hematopoietic stem and progenitor ce...
I hope this helps :)
Valeria Tananska
Thank you very much.
I'll take a good look at all the sources you mentioned.
Best regards,
Daisuke
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