Daisuke Koga

10 Questions 18 Answers 0 Followers

Questions related from Daisuke Koga

Should all pipette tips be pyrogen-free grade?

I'm selecting the pipette tips to use in our new lab. We are going to buy bulk tips and use them after autoclaving them. I found that some products are described as "pyrogen-free" and some are...

03 October 2024 121 2 View

How to detect ssDNAs with acrylamide gel electrophoresis?

I'm trying to detect ssDNAs with acrylamide electrophoresis, which seems not working well. I loaded 10 uL of single strand oligo DNA whose length and concentration is 20 nt and 100 uM, which...

19 September 2024 5,863 8 View

What to use: Anesthesia for bone marrow transplantation in mice ?

Could anyone recommend the appropriate anesthetic procedure for BMT in mice? I do bone marrow transplantation in mice as a part of my study. The problem is, the engraftment rate got pretty low...

27 March 2021 9,728 5 View

Premix the antibodies for FACS?

Is it possible to premix and store multiple antibodies for FACS? I usually use more than 5 colors on FACS, which consist of around 10 antibodies. It's kind of a hassle mix all the antibodies for...

13 May 2020 6,375 0 View

Ser2 and Ser5 in CTD of RNA polymerase ii : the order of count?

Which of Ser2 and Ser5 on the C-terminal domain of RNA polymerase ii is supposed to be located on the C-terminal side? I saw a figure in which Ser2 is drawn on the C-terminal side of Ser5, but it...

29 April 2020 7,004 0 View

Maximum size of the sequence deleted with CRIPSPR/Cas9 ?

I'm designing sgRNAs to delete a fraction of a gene by cutting two sites with CRISPR/Cas9 system. I'm wondering how big the fragment deleted can be. Is there a recommended maximum length of the...

09 March 2020 2,917 9 View

Appropriate concentration of fibronectin to coat plates for HSCs culture?

I'm trying to culture hematopoietic stem cells of mice, referring to an article, in which fibronectin-coated plates are used. Since pre-coated plates are expensive, I'm thinking about coating...

29 August 2019 2,783 2 View

What's the Sca-1(+), c-Kit(–) popultation of murine hematopoietic progenitor?

Does anyone know what's the Lin(–), c-Kit(–), Sca-1(+) population of murine hematopoietic progenitors? I use murine HSCs and sort them by FACS, and of course, my major interest is Lin(–),...

23 August 2019 6,021 4 View

Need more cells for bone marrow transplant via tail vein than viaretro-oribital sinus?

I'm practicing the bone marrow procedure on mice, and the problem is the engraftment rate is poor. I collect BM cells from long bones of mice and make a suspension fluid directly, which means I...

08 July 2019 1,036 6 View

How to check if my new vector works properly by using Tamoxifen dependent conditional KO in ES cells?

I'm a 1st year graduate student. I've just constructed a new retrovirus vector which holds CreERT2 system. Now I'm considering about checking it's function by transfecting it to ES cells which has...

10 June 2019 2,260 3 View