Daisuke Koga

6 Questions 8 Answers 0 Followers

Questions related from Daisuke Koga

What to use: Anesthesia for bone marrow transplantation in mice ?

Could anyone recommend the appropriate anesthetic procedure for BMT in mice? I do bone marrow transplantation in mice as a part of my study. The problem is, the engraftment rate got pretty low...

27 March 2021 9,668 5 View

Premix the antibodies for FACS?

Is it possible to premix and store multiple antibodies for FACS? I usually use more than 5 colors on FACS, which consist of around 10 antibodies. It's kind of a hassle mix all the antibodies for...

13 May 2020 6,327 0 View

Ser2 and Ser5 in CTD of RNA polymerase ii : the order of count?

Which of Ser2 and Ser5 on the C-terminal domain of RNA polymerase ii is supposed to be located on the C-terminal side? I saw a figure in which Ser2 is drawn on the C-terminal side of Ser5, but it...

29 April 2020 6,958 0 View

Maximum size of the sequence deleted with CRIPSPR/Cas9 ?

I'm designing sgRNAs to delete a fraction of a gene by cutting two sites with CRISPR/Cas9 system. I'm wondering how big the fragment deleted can be. Is there a recommended maximum length of the...

09 March 2020 2,888 9 View

Appropriate concentration of fibronectin to coat plates for HSCs culture?

I'm trying to culture hematopoietic stem cells of mice, referring to an article, in which fibronectin-coated plates are used. Since pre-coated plates are expensive, I'm thinking about coating...

29 August 2019 2,741 2 View

How to check if my new vector works properly by using Tamoxifen dependent conditional KO in ES cells?

I'm a 1st year graduate student. I've just constructed a new retrovirus vector which holds CreERT2 system. Now I'm considering about checking it's function by transfecting it to ES cells which has...

10 June 2019 2,227 3 View