EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

After staining a gel with coomassie can I stain the same gel with fluorescent dye?

I want to know if it is possible to be done the inverse to view / verify other bands that not look in staining a gel with coomassie.

01 February 2021 8,647 5 View

Permanent Fluid temperature initialization in Ansys Fluent?

Dear researchers, I am coupling Design Optimization with ANSYS Fluent in Workbench 2020 R2. I can do the analysis. I am patching the initial Temperature values of the zones. However, the patch...

28 January 2021 4,265 2 View

Luciferase assay for miRNA-gene: How to obtain DNA (PCR product) from the mutated primer?

To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...

19 January 2021 8,568 3 View

How to couple Moldex 3D with MATLAB for design optimization of an injection mould?

Hello, I would like to use the software Moldex 3D for the design optimization of an injection mould. How can I couple Moldex 3D with a MATLAB code? Any help appreciated, I wish you all a happy...

09 January 2021 569 2 View

How is the quality of archived blood sample for HbA1c test?

HbA1c is an important indicator of long-term glycemic control with the ability to reflect the cumulative glycemic history of the preceding two to three months. Also, HbA1c is measured as the ratio...

16 September 2020 2,423 4 View

Any mathematical study for sickle cell disease?

Any mathematical study for sickle cell disease?

21 February 2020 4,857 4 View

Which biochemical modification of Hemoglobin can potentially carry more oxygen to bloodstream?

Any synthetic analogue of Hemoglobin or any other Xenobiological agents can be cited as example. Please provide links and references.

20 November 2019 8,904 4 View

Which biochemical modification of Hemoglobin can potentially carry more oxygen to bloodstream?

Any synthetic analogue of Hemoglobin or any other Xenobiological agents can be cited as example. Please provide links and references.

10 November 2019 2,970 4 View

There is an alternative to sodium dithionite as a reducing agent in the Hemoglobin Assay measuring NO?

Hello, I'm trying to reduce He to HeO2 with Sodium Dithionite, protocol for measuting nitric oxide from the Hemoglobin assay (Murphy and Noak, 1994). But for some reason (my suspicion is a bad...

09 October 2019 761 3 View

What could cause this side peak during Drabkin's Reaction?

Hey there, I'm using commercially available Drabkin's reagent to measure the hemoglobin content of blood photometrically. The reagent I bought contains sodium bicarbonate, potassium ferricyanide...

09 October 2019 1,497 10 View

Inducer for low oxygen polymerization of normal hemoglobin?

Hemoglobin of sickle cell patients polymerizes at low oxygen condition. Does anyone know of a way to simulate this process of reversible, oxygen-dependent, hemoglobin polymerization?

07 August 2019 3,084 3 View

What is the single sensor where we can measure temperature, heart rate, blood pressure, oxygen saturation and hemoglobin noninvasively?

Which sensor can measure all the parameters mentioned above noninvasively?

04 May 2019 5,511 2 View

SDS-PAGE of Hemoglobin?

I am receiving bands between 10 to 15kda of hemoglobin on SDS PAGE. Each subunit of hemoglobin is 16kda. What should be the problem? 12% resolving gel 4% Stacking gel.

03 April 2019 239 4 View

No title

03 April 2019 8,101 2 View