Ambion (now Thermo Fisher) offers DNase removal reagent. What’s the recipe for this reagent?
CIR 2021/808 written as "the CCα shall be calculated as follows: Method 1: by the calibration curve procedure according to ISO 11843-1:1997 ( 14) (here referred to as critical value of the net...
13 May 2024 9,481 0 View
would like to use the VSH 15-item tool to assess sleep quality, but I'm unsure where to obtain approval for its use. I understand that the developer of the tool has passed away. Could you please...
03 May 2024 609 0 View
Assuming there's no detected contamination in the cabinet, how often should I sterilize the CO2 cabinet? Co-worker suggested we should maintain an absolute clean surroundings (to the...
18 March 2024 5,900 0 View
I'm currently working with animal tissue, more specifically visceral fat tissues from dogs. Our labs are not equipped to function 24/7 at the moment so I have to make sure the sample collection...
25 February 2024 2,835 4 View
Are ethics the domain of humans only? Can AI philosophize? Thanks
22 October 2023 3,262 6 View
Please refer to the photo attached. I am currently trying to determine my ion-exchange membrane (IEM)'s ionic conductivity with Potentiostatic electrochemical impedance spectroscopy (PEIS) with a...
29 August 2023 5,952 17 View
After I login my account and found my paper, Then click the title of the paper and from "more" to choose "edit". Unfortunately only some meta part of the paper appeared, thus I can't correct the...
03 June 2023 6,721 1 View
Click the title of paper-from "more" to "edit"-only some meta part of paper appears-----the author can't correct the details of the paper!!!
03 June 2023 4,919 2 View
What is a narrative case study? Is it by nature a case study, but with some narrative features or elements? For example, by highlighting narrative knowledging or by adding story constellations...
13 May 2023 4,934 2 View
Hi, dear friends online. I am a fresh person in bioinformatics with little understanding. but I need to learn to analyze RNA-sequencing data. I think python is simple, I want to use it to...
05 April 2023 2,626 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View
So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...
14 July 2024 641 1 View
I have a small molecule reagent with a boc-protected amino group. Now the reaction needs to be reacted at 37°C for 30 h. Is this protection group stable?
12 July 2024 3,745 2 View
Please explain the protocol in detail along with the reagents to be used
03 July 2024 876 1 View