I'm currently working with animal tissue, more specifically visceral fat tissues from dogs. Our labs are not equipped to function 24/7 at the moment so I have to make sure the sample collection time, the live tissue refrigeration period and tissue viability is within range of each other.
My questions are:
1. What is the best range for general tissue refrigeration period (for mammal tissue)?
2. Could I use any salt-based balance solution to help ensure tissue viability after collection?
at 4 degrees, in absence of any support solution and oxygen/CO2... I do not think you could expect a long duration... maybe less than 1 hour? It really depends on the tissue and its metabolic demands. Brain tissue would be dead in no time.
The viability of live tissue outside the body depends on several factors, including the type of tissue and the conditions of preservation. In general, most tissues start to deteriorate rapidly once removed from the body. For refrigeration at 4℃ (39.2°F), you might expect some preservation for a limited time, typically a few hours, but this can vary based on the specific tissue.
Thank you all for your recommendations! I'll come back for an update if the samples somehow survive longer than we'd expected
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