Are you injecting the mouse or providing the dissolved substance by mouth? if by injection, IV or Intraperitoneal (IP) injection? For IP, i routinely use 50 microliter injections.

Ehanol is used at a max concentration of 5% for formulations with cremophor or DMSO, for instance and it may be used every day. This suggests that a mouse of 20 gr can receive a maximum amount of ethanol (10 mg) if you use a standard volume of 10 mL/kg.

The mice will be like around 16g or even less as the experiments goes,

and total 200ul will be fed to them, so 10ul ethanol per day would be fine?

http://www.afstal.com/medias/File/Ethical/Techniques/Nonclinical%20vehicle%20use%20PDF.pdf

Good reference

For IP injection maximun volume @10ml/kg bwt can be given so if  mice wt is 20gm maximum volume IP you can give 0.2ml.

Hi! Does someone know the values for maximum subcutaneous injection volume of ethanol in mice?

70 % Ethanol is well tolerated up to 5 ml/kg dose volume by cutaneous route. (100 μl/20 gram mouse/day).

We may inject this volume in repeated days also.

Thank you for the valuable info.

What is the tolerability by oral administration? Max volume and percentage of ethanol?

What would be the concentration v/v, if we use ethanol as a solvent for intratracheal drug delivery

Hi Atul Kansagara !

Do you have any references (I mean published papers) for your answer (70 % Ethanol is well tolerated up to 5 ml/kg dose volume by cutaneous route. (100 μl/20 gram mouse/day) please ?

Is there any article for intravenous injection by taking ethanol as vehicle?

http://www.afstal.com/medias/File/Ethical/Techniques/Nonclinical%20vehicle%20use%20PDF.pdf

Hi Loredana Vesci!

Can you share the reference regarding " a max concentration of 5% for formulations with cremophor or DMSO, " thanks a lot!

This reference about the tolerability of solvents for intravenous injection may be helpful.

Article Solvent-based formulations for intravenous mouse pharmacokin...