whats the exact method to do the MIC test of Malasseziafurfur?
Can we do it in SD broth with 1% olive oil? Or is it mandatory to do it on agar? Which s the best? Can someone share the exact protocol in pdf?
If ethanol has analgesic properties, why isn't it used for clinical purposes?
18 February 2021 8,227 4 View
It takes a lot of time in publishing the paper in the journal, from few months to a year so how one can able to decrease the time for publication.
17 February 2021 2,997 14 View
Hi, Can we use FDD algorithm for forced vibration response (sinusoidal response produced from eccentric mass vibrator)? Thanks
22 January 2021 5,851 2 View
Hello, For now, I am following the integrated technique to derive the mass from the smaller scale (microstructure). Is there any better approach to get the mass of a given macro-structure from...
16 January 2021 4,792 1 View
I am going to start my master program (msc agri hons) research in rull of potassium in tomato vigetable crop so what should my objective please guide me Thanks
13 January 2021 4,635 6 View
how do you understand life as it is really big suspense, why we are here, our consciousness is big unconsciousness and our unconsciousness big consciousness.
25 December 2020 7,632 19 View
Many time during meditation whole body feels the flow of electricity like the thing is it bio electricity or something else why this occurs but after that generally, I felt so miracle...
25 December 2020 3,754 3 View
we all have our own thought and experience regarding yoga and meditation, we all know it's a miracle unexplainable to those who do he/she feel it.What's your view regarding it.
24 December 2020 3,615 10 View
we all know that it is our traditional practice which we are performing in any problem in our kitchen garden to farm level is it good to apply or not, how it is good or what are its effects.
24 December 2020 2,699 15 View
In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.
24 December 2020 4,068 6 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...
17 February 2021 7,029 3 View
I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...
16 February 2021 169 4 View