What are the options for testing the phosphorylation of HP1a?

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What's the concentration recommend for recombinant protein use in western blotting?

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Adam B Shapiro

If you are using purified proteins, the simplest method (if you can find a lab that has the necessary equipment) to detect phosphorylation would be intact protein mass spectrometry, either by electrospray or MALDI. Those methods would easily distinguish the mass change due to addition of phosphate. No antibodies needed.

Xupiter Monk

Adam B Shapiro Thanks. I believe that would be the best option.