I have used CSII to phosphorylate HP1a, in vitro. Now, to test this phosphorylation, I have options such as using an anti-serine phospho antibody or mass spectrometry.
Has anyone ever tested an anti-serine phospho antibody western blot that worked for them?
Any recommendations and catalog numbers would be helpful.
Adam B Shapiro
If you are using purified proteins, the simplest method (if you can find a lab that has the necessary equipment) to detect phosphorylation would be intact protein mass spectrometry, either by electrospray or MALDI. Those methods would easily distinguish the mass change due to addition of phosphate. No antibodies needed.
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