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Suppose I use "pBABE-puro-GFP-wt-GeneX" to get GeneX expressing with GFP (I am not sure whether it will replace the endogenous GeneX or get inserted somewhere else since the pBABE-puro vector uses...
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Can anyone share their experience regarding the acquisition or gifting of cell lines mentioned in a Nature article? Specifically, I am interested in knowing if there are any journal-specific...
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Should I use multiple gRNA transfections (Donor) mixed as well as one by one along with a Cas9 plasmid to see which gRNA is working, or start with only 1 gRNA at a time? Options: 1 gRNA + Cas9 (1...
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I have used CSII to phosphorylate HP1a, in vitro. Now, to test this phosphorylation, I have options such as using an anti-serine phospho antibody or mass spectrometry. Has anyone ever tested an...
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I have a couple of proteins (95-130 KDa) to make them clean. What are the best microcentrifuge tube based column filtrations you guys have tried and worked best. Any suggestion with direct product...
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Does anyone know if CMV or EF1a which is better to express gene by Safe Harbor CRSPR knock in U2OS cells. Some cell line doesn’t express through CMV promoter in some cell lines. Is it the case for...
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If we do FRET in solution and there could be intra molecular FRET possible. Is there minimal concentration recommended for the protein to avoid this or there is any way to avoid intramolecular...
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