Usually random hexamers give the desired results. but I mistakenly added oligo dT primers also.
Did you add the same quantity of random hexamers as usual, AND ALSO some oligo d(T)? I am fairly sure it will make no difference to your qPCR results, but it would be best practice to avoid direct comparison of these samples to other samples without the dT primers for important experiments/publication.
If you added less random hexamers than usual, then you might get some wonky results. This is mainly because reducing the concentration of random hexamers would likely reduce your cDNA yield, and also changing the ratio of random hexamers to cDNA would affect the size distribution / average length of the cDNA. You would be able to get a reasonable idea how different it was by comparing the absolute cT values for reference gene/s between reactions set up with your usual cDNA and the cDNA with both primers in it.
For the benefit of future readers who have made the opposite error (normally use dT, added hexamers in error) - this mistake is likely incompatible with the experiment depending on the reason for using dT priming only. If you are trying to create full-length cDNA or avoid reverse transcription of pseudogene transcripts or similar, probably start again.
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