Sadab Khan

5 Questions 7 Answers 0 Followers

Questions related from Sadab Khan

Anyone working on RNA from Tears and downstream apllications like bacterial 16S qRT-PCR?

I am working on quantifying different bacterial 16S gene expression from Human Tear RNA and its comparison with universal 16S RNA. I am not getting proper quantification. any suggestions would be...

26 September 2023 9,991 2 View

Can anyone suggest me from where can i order or procure Lactobacillus Selection media Agar plates for selective isolation of Lactobacillus sp.?

i need a media for the selective isolation of Lactobacillus sp.

29 May 2023 6,607 4 View

What would be the negative impact of adding both oligo dT and random hexamers primers in cDNA conversion from RNA extracted from ocular tears?

Usually random hexamers give the desired results. but I mistakenly added oligo dT primers also.

03 March 2023 4,327 1 View

Housekeeping genes in bacteria

Can anyone suggest common housekeeping genes or stress/adhesion genes common to all bacteria that can be used to quantify and compare stress due to any external factor between different bacterial...

01 January 1970 9,254 1 View

Detection of bacterial presence by realtime PCR

Can anyone comment or suggest how to standardize bacterial 16S realtime PCR primers for detecting bacterial presence/absence through qPCR?

01 January 1970 6,266 2 View