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Questions related from Maurice Overzier
Currently I'm culturing primary human fibroblasts and keratinocytes from a skin punch in medium (DMEM with 20% FBS and 1% P/S). I would like to culture these cells separately, but I was wondering...
02 March 2020 9,584 3 View
A while ago the website of Qvotek went offline, and their supplies haven't been open for ordering. We were using their custom 5 micron micropipettes/needles/tips for microinjections in zebra fish...
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As stated in the question, I would like to know what kind of culturing medium is generally used for culturing primary human VSMC. Currently for murine cells I'm using DMEM with 10% FBS, Pen/Strep,...
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For capturing blood vessels we are using acid washed glass beads from Sigma (G8772). Although, after autoclaving, these beads tend to stick together and are hard to remove from the glass wear in...
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