Dear all,
We cultivated E. Coli in LB Agar in 6 plate which contain respectively sample itself, 1/10 diluted sample, 1/100, 1/1.000 and 1/10.000 . Later we incubated plates at 37oC for 24h. When we take plates out off incubator we saw that 1/1.000 and 1/10.000 diluted sample have showed very dense growth. Even the sample itself was more able to count while the other 2 plate was impossible to count. Is there any one who think of why would such think happened and what would be the cause?
Best regards,
Hatice
Hello Hatice, Francoise may be right.
-Have you tried using a control (not inoculated) plate?
-It could be a mixed culture, some species do better in low E. coli concentrations
--Attempt to transfer ONE "normal" E. coli colony onto a new plate. Then make an E. coli broth culture for that dilution procedure.
I hope you find what you're looking for, take care!
Hi all, First how you cultivated using broth medium in plates!!!!! This may cause a contamination with high percentage. you should use tubes for broth media and make replicates for each dilution, I think this will avoid contamination and give you real results.
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