Global change in protein expression?

Dear all, When I run my yeast total protein lysate extracted after 10 minute-treatment with my compound, I observe a global up regulation in proteins, even my loading control protein. I suspect...

03 January 2021 1,388 8 View

Tetracycline/doxcycline treatment?

Hi all, I am not sure whether or not this question was asked. I wonder can I induce expression of my protein with tetracycline or doxycyxline while seeding cells instead of one day after seeding...

05 November 2020 2,417 4 View

What is the minimum amount of carotenoid for 600 MHz H and C NMR analysis in characterization?

We are trying to isolate some carotenoids from algae with semi-prep HPLC. As literature says, a few mg will be enough. Is this true?

07 July 2020 8,024 9 View

Calcium Phosphate Transfection Problem?

Hi, I am trying to transfect 293T cells using 2M CaCl2 and 2X HBS (50 mM HEPES, pH 7.07, 10 mM KCl, 12 mM dextrose, 280 mM NaCl, 1.5 mM Na2PO4) However, some plates, still have particles (probably...

23 March 2018 5,856 7 View

What would be the cause of high density growth in 1/1000 and 1/10.000 diluted LB Agar?

Dear all, We cultivated E. Coli in LB Agar in 6 plate which contain respectively sample itself, 1/10 diluted sample, 1/100, 1/1.000 and 1/10.000 . Later we incubated plates at 37oC for 24h. When...

24 February 2018 5,372 3 View

How to find in ANSYS ALPD and BETD value?

Hello everyone, I am using ansys but I am somewhere I have calculated ALPAD and BETAD values but I want to enter ALPD and BETD values in its material. how to find these values in ANSYS.Thanks in...

21 January 2018 8,584 1 View

SiRNA Transfection to COLO205, SW620, HT29?

Hi, Is there anyone who has done siRNA Transfection in COLO205, SW620, HT29 especially using either RNAiMAX or Lipofectamine3000? Besides do you have any experience to transfect/transduce plasmid...

04 December 2017 2,369 3 View

Is there any criteria for antioxidant rich food products ?

There are lots of literature datas for antioxidant rich products. But i have a question there are any limits to be able to call a food as a antioxidant rich product. When i compare fruits and...

15 May 2017 1,863 4 View

What is the duration of 30% sucrose preservation for uterus tissue?

Does anyone know that how long we should keep uterus tissues in %30 sucrose before cryopreservation or before sectioning?

19 October 2016 8,472 1 View

Normalization for Immunoprecipitation?

07 June 2016 5,408 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

No title

22 February 2021 9,932 11 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

How to select therorical or conceptual framework for any proposed qualitative research work?

Hi, I am conducting an exploratory research with qualitative in-depth interview approach to understand the impact of COVID_19 in health systems response to burn care in India. Since, this is a...

19 February 2021 4,562 4 View

How to model rupture in geogrid in abaqus?

Hi researchers I wanna model tensile test on geogrid in abaqus, so I added displacement to one side of geogrid and the other side of geogrid is constrained completely, everything seems normal but...

19 February 2021 2,195 2 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

When to use different framework?

Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...

18 February 2021 9,581 2 View