I would like to combine ANI and AAI metrics to study genus and species frontiers among a set of genomes (a total of 182). I got ANI output by using fastANI; however, I am having some trouble with AAI metrics. I tried with enveomics software, but for some reason, I am not able to open the program for more than 2 minutes straight.

Now, I am trying with EzAAI but I am getting java errors all the time, I guess I should be able to figure it out, but in case not, is there another alternative out there?

Best regards,

Ana

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