20 September 2017 2 960 Report

Using this system for the 1st time.

(a)From what I read from other posts, many suggested retaining the ccdB gene  but using a ccdB resistant strain will help in cloning of the control plasmid. Would you sugest any other plasmid?

 (b)Assuming I am retaining the ccdB gene as my control gene, should I use the plasmid or different( eg pGWB5 for both gene of interest and control OR pGWB5 for gene of interest and pGWB20 for control)?

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