Using this system for the 1st time.
(a)From what I read from other posts, many suggested retaining the ccdB gene but using a ccdB resistant strain will help in cloning of the control plasmid. Would you sugest any other plasmid?
(b)Assuming I am retaining the ccdB gene as my control gene, should I use the plasmid or different( eg pGWB5 for both gene of interest and control OR pGWB5 for gene of interest and pGWB20 for control)?