Recently I've been looking T CD4 subset and the CBA kit of BD Th1/Th2/Th17 hasn't been enough for my needs, I want to investigante another subsets such as Th9/Thf. Biolegends has this similar beads assay to evaluate cytokine with more cytokine such as IL-9 and IL-21, but I don't have experience with this kit and my research colleges never used this kit. Does anyone has a experience with this kit? Does it perform well with ex vivo splenocyte supernatant?
Thanks
I have experience using multiplex ELISA kits from BD and BioLegend and both work great. I started with BD CBA kits but like you, switched to BioLegend due to the additional cytokines. If you reach out to your local BioLegend representative, you may be able to get a sample kit to try it out first.
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