I am trying to find out what will be the effects of microplastics in cells and for that, I will use a fluorescent microplastic to act as a stressor and then measure ROS by fluorescence.
I will perform two analyses:
First using a fluorometer I will observe microplastic intensity and then ROS intensity.
Second, using fluorescence microscopy I will observe there is colocalization of the fluorescent microplastic and the local increase of ROS production.
In both assays which is the best Fluorescent microplastic to use, Green (480nm emission/ 501nm fluorescence) or Orange (475nm emission/ 540nm fluorescence ), if the H2DCFDA has a fluorescence of 488nm /525nm?
PS. I cannot use a Red or blue-dyed microplastic as it overlaps with other tests
It is interesting to learn that you can buy fluorescent microplastics. Do they differ from the fluorescent latexes that have been used for years as nano sensors and to study endocytose and phagocytose ? Did you find bibliographic evidence of ROS production in cell against these latexes ,
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