What type of purification technique and resin is best to purify cytochrome p450?

More Gajula Hari's questions See All

On 27 May 2024, accidentally I removed 25 papers from my list of publications on Rsearch Gate. How to restore deleted papers?

28 May 2024 3,927 1 View

Explain the geological cycle of formation of different types of rocks .what are the physical ,chemical,and biological phenomenon involved in forming?

need answer in a tleast 20 lines

21 April 2024 5,489 0 View

I treated CNTs in reduced Nickel chloride solution using sodium boro hydride. After centrifuing i got mixture of CNT and nickel. how to seperate CNT?

I treated CNTs in reduced Nickel chloride solution using sodium boro hydride. After centrifuing i got mixture of CNT and nickel. how to seperate CNT? i have done this for decorating CNTs wit...

20 April 2024 6,574 1 View

How to create titianium metal nanoparticles from titanium tetrachloride ?

using solution based method

17 November 2023 8,243 1 View

Any research people wil help me in docking studies? I have chemistry work???

Iam Dr M HARIKRISHNA doing research in organic chemistry, I need help for docking studies?

03 November 2023 9,336 3 View

I want to measure the work function of fused silica. Is it possible to measure the work function using UPS? Does anyone have experience?

I want to measure the work function of fused silica. Is it possible to measure the work function using UPS? I have seen several papers using UPS, but did not get the concept behind that. Does...

02 November 2023 3,779 4 View

how to find out volume of chamber 2, using the equation state of an ideal gas by isothermal expansion of a known volume of a gas from chamber1 to 2?

how can we find out the volume of chamber 2, using the equation state of an ideal gas by isothermal expansion of a known volume of a gas from chamber 1 to chamber 2

01 November 2023 7,811 0 View

Why MXene and MoS2 partcles can not show proper EDS results when mix with PDMS ?

Dear Researches I have a some doubt,recently I am working on fabrication of PDMS-based composites. My question is when I mix MXene and SrTiO3 like particles with PDMS and make a cast film....

01 August 2023 1,928 2 View

What are the best methods for grinding PLA pellets?

I need to grind PLA in order to perform hydrolysis with a liquid ionic catalyst. So far, all I have tried is grinding the pellets in a small coffee/spice grinder along with dry ice for cooling....

27 June 2023 4,762 1 View

Being new to this i have some few questions regarding TGA analysis. I can describe that?

1. While am checking the TGA results, i found three columns such as temperature, weight %, Deriva: weight %/ degree celcius. In this the column Deriva: weight % per degree celcius belongs to...

21 June 2023 1,787 1 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View